奶牛源金黄色葡萄球菌新疆流行株的耐药特性、毒力基因及分子分型

doi:10.11843/j.issn.0366-6964.2018.01.021

Abstract

Abstract:

In order to know the antimicrobial resistance, virulence genes and the epidemiological characteristics of Staphylococcus aureus (SA) isolated from cows with mastitis and endometritis. The drug resistance profile for 155 clinical S. aureus isolates were determined using disk diffusion method, and methicillin-resistant Staphylococcus aureus (MRSA) strains were also identified. Antimicrobial genes, virulence genes, SCCmec genotyping and MLST genotyping were detected and analyzed by using PCR method. The results showed that a total of 22 MRSA strains have been detected among 155 SA isolates, with the detection rate of 14.2%. The antimicrobial resistant rates of most MRSA were significantly higher than that of the MSSA strains; and the detection rates of different virulence genes were different between MRSA and MSSA. The SCCmecⅠwas identified as a predominant genotype of SCCmec in Xinjiang region. Fourteen ST genotypes were detected, namely ST188, ST584, ST9, ST805, ST2373, ST968, ST2139, ST1, ST2700, ST903, ST2454, ST2990, ST63 and STX, respectively. ST1 and ST9 had higher detection rates than others. ST9 was higher among MSSA strains and ST1 was higher among MRSA strains. This study showed that SA strains were of multiple resistance with diverse distribution of virulence genes. While ST9 was the predominant genotype among MSSA strains and ST1-SCCmecⅠwas the predominant genotypes among MRSA strains in Xinjiang region.

CLC Number:

S852.611

MENG Dan, MENG Qing-ling, QIAO Jun, CAI Kuo-jun, WANG Deng-feng, MA Shuai, WU Ye-hui, CAI Xue-peng. Study on Resistance Characteristics, Virulence Genes and Molecular Classification of Staphylococcus aureus Isolated from Cows in Xinjiang[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(1): 181-194.

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Study on Resistance Characteristics, Virulence Genes and Molecular Classification of Staphylococcus aureus Isolated from Cows in Xinjiang

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